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bovine serum albumin galnac  (Vector Laboratories)


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    Vector Laboratories bovine serum albumin galnac
    16A mAb binds to both sugar and peptide parts of a MUC1 glycopeptide. The biotinylated glycopeptide, <t>RPAPGS(GalNAc)TAPPAHG-dPEG™11-Biotin,</t> (1 μg/ml) was bound to streptavidin-coated plates (2 μg/ml) and incubated with 16A monoclonal Ab (mAb) for 2 h. Binding of 16A was visualized by a secondary Ab (goat anti-mouse IgG) followed by colorimetric detection. To measure the inhibitory effects of competing ligands, <t>ligands</t> <t>(GalNAc-BSA,</t> GalNAc, RPAPGS(GalNAc)TAPPAHG, and RPAPGSTAPPAHG) were mixed with the 16A mAb at 0 to 500 μM for 1 h, before incubation with plate-bound glycopeptide RPAPGS(GalNAc)TAPPAHG-dPEG. Data were representative of 3 independent experiments.
    Bovine Serum Albumin Galnac, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine serum albumin galnac/product/Vector Laboratories
    Average 96 stars, based on 1795 article reviews
    bovine serum albumin galnac - by Bioz Stars, 2026-02
    96/100 stars

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    1) Product Images from "Molecular basis of antibody binding to mucin glycopeptides in lung cancer"

    Article Title: Molecular basis of antibody binding to mucin glycopeptides in lung cancer

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2015.3302

    16A mAb binds to both sugar and peptide parts of a MUC1 glycopeptide. The biotinylated glycopeptide, RPAPGS(GalNAc)TAPPAHG-dPEG™11-Biotin, (1 μg/ml) was bound to streptavidin-coated plates (2 μg/ml) and incubated with 16A monoclonal Ab (mAb) for 2 h. Binding of 16A was visualized by a secondary Ab (goat anti-mouse IgG) followed by colorimetric detection. To measure the inhibitory effects of competing ligands, ligands (GalNAc-BSA, GalNAc, RPAPGS(GalNAc)TAPPAHG, and RPAPGSTAPPAHG) were mixed with the 16A mAb at 0 to 500 μM for 1 h, before incubation with plate-bound glycopeptide RPAPGS(GalNAc)TAPPAHG-dPEG. Data were representative of 3 independent experiments.
    Figure Legend Snippet: 16A mAb binds to both sugar and peptide parts of a MUC1 glycopeptide. The biotinylated glycopeptide, RPAPGS(GalNAc)TAPPAHG-dPEG™11-Biotin, (1 μg/ml) was bound to streptavidin-coated plates (2 μg/ml) and incubated with 16A monoclonal Ab (mAb) for 2 h. Binding of 16A was visualized by a secondary Ab (goat anti-mouse IgG) followed by colorimetric detection. To measure the inhibitory effects of competing ligands, ligands (GalNAc-BSA, GalNAc, RPAPGS(GalNAc)TAPPAHG, and RPAPGSTAPPAHG) were mixed with the 16A mAb at 0 to 500 μM for 1 h, before incubation with plate-bound glycopeptide RPAPGS(GalNAc)TAPPAHG-dPEG. Data were representative of 3 independent experiments.

    Techniques Used: Incubation, Binding Assay



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    16A mAb binds to both sugar and peptide parts of a MUC1 glycopeptide. The biotinylated glycopeptide, RPAPGS(GalNAc)TAPPAHG-dPEG™11-Biotin, (1 μg/ml) was bound to streptavidin-coated plates (2 μg/ml) and incubated with 16A monoclonal Ab (mAb) for 2 h. Binding of 16A was visualized by a secondary Ab (goat anti-mouse IgG) followed by colorimetric detection. To measure the inhibitory effects of competing ligands, ligands (GalNAc-BSA, GalNAc, RPAPGS(GalNAc)TAPPAHG, and RPAPGSTAPPAHG) were mixed with the 16A mAb at 0 to 500 μM for 1 h, before incubation with plate-bound glycopeptide RPAPGS(GalNAc)TAPPAHG-dPEG. Data were representative of 3 independent experiments.

    Journal: International Journal of Oncology

    Article Title: Molecular basis of antibody binding to mucin glycopeptides in lung cancer

    doi: 10.3892/ijo.2015.3302

    Figure Lengend Snippet: 16A mAb binds to both sugar and peptide parts of a MUC1 glycopeptide. The biotinylated glycopeptide, RPAPGS(GalNAc)TAPPAHG-dPEG™11-Biotin, (1 μg/ml) was bound to streptavidin-coated plates (2 μg/ml) and incubated with 16A monoclonal Ab (mAb) for 2 h. Binding of 16A was visualized by a secondary Ab (goat anti-mouse IgG) followed by colorimetric detection. To measure the inhibitory effects of competing ligands, ligands (GalNAc-BSA, GalNAc, RPAPGS(GalNAc)TAPPAHG, and RPAPGSTAPPAHG) were mixed with the 16A mAb at 0 to 500 μM for 1 h, before incubation with plate-bound glycopeptide RPAPGS(GalNAc)TAPPAHG-dPEG. Data were representative of 3 independent experiments.

    Article Snippet: Bovine Serum Albumin-GalNAc (each BSA carries 23 GalNAc residue) were from Vector Labs (UK).

    Techniques: Incubation, Binding Assay